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iq5 qpcr machine  (Bio-Rad)


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    Bio-Rad iq5 qpcr machine
    Iq5 Qpcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iq5+qpcr+machine/cfx96+touch+real+time+pcr+detection+system/pm39091151-106-14-17
    Average 90 stars, based on 1 article reviews
    iq5 qpcr machine - by Bioz Stars, 2026-09
    90/100 stars

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    Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time <t>quantitative</t> <t>PCR</t> data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.
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    Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time quantitative PCR data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.

    Journal: The American Journal of Pathology

    Article Title: Bromodomain and Extraterminal (BET) Proteins Regulate Hepatocyte Proliferation in Hepatocyte-Driven Liver Regeneration

    doi: 10.1016/j.ajpath.2018.02.006

    Figure Lengend Snippet: Bromodomain and extraterminal (BET) inhibition reduced hepatocyte proliferation in the zebrafish acetaminophen (APAP)-induced injury model. A: Scheme illustrating the periods of APAP and JQ1 treatments and analysis stages (arrows). B: Confocal images showing the hepatic expression of fabp10a:CFP (gray, hepatocytes), Tp1:H2B-mCherry (red, biliary epithelial cells), and fabp10a:mAGFP-gmnn (green, proliferating hepatocytes). C: Quantification of the number of mAGFP-gmnn+ cells, as shown in B. D–H: Real-time quantitative PCR data showing the relative expression levels of ccnd1, pcna, myca, brd4, and axin2 among uninjured control livers at 5 days after fertilization (dpf) and dimethyl sulfoxide (DMSO)- and JQ1-treated regenerating livers at 12 hours of recovery (R12h). Data are expressed as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001 (one-way analysis of variance). Scale bar = 100 μm. CFP, cyan fluorescent protein.

    Article Snippet: Real-time quantitative PCR (qPCR) was performed as previously described, 33 using the Bio-Rad iQ5 qPCR machine with the iQ SYBR Green Supermix (Bio-Rad).

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Control